After Joining Rice: equal contribution; * corresponding author; # undergraduate student.

27. Lee, S.S.†, Ding, N.†, Sun, Y.D, Yuan, T.L., Li, J., Yuan, Q.C., Liu, L.Z., Yang, J., Wang, Q., Kolomeisky, A.B., Hilton, I.B, Zuo, E.W.*, Gao, X.*,“Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome- and transcriptome-wide off-target effects.” Sci. Adv., 2020,

‘Bystander’ Cs meet their match in gene-editing technique

Base Editor Converts “Wanted” Cs, Spares “Bystander” Cs

26. Zhao, F.L., Liu, Z.W., Yang, S.Y., Ning, D., Gao, X.*,Quinolactacin Biosynthesis Involves NRPSs Catalyzed Dieckmann Condensation to Form the Quinolone‐γ‐lactam Hybrid.” Angew. Chem. Int. Ed., 2020, (VIP Paper)

Engineers enlist fungi to advance against disease

25. Liu, W.W., An, C.Y., Shu, X., Meng, X.X., Yao, Y.P., Zhang, J., Chen, F.S., Xiang, H., Yang, S.Y., Gao, X.*, and Gao, S.S.*, “A dual-plasmid CRISPR/Cas system for mycotoxin elimination in polykaryotic industrial fungi.” ACS Synth. Biol., 2020,

24. Ding, N., Lee, S.S., Lieber-Kotz, M.#, Yang, J., Gao, X.*, “Advances in Genome Editing for Genetic Hearing Loss.” Adv. Drug Deliv. Rev., 2020,

23. Wan, T., Chen, Y., Pan Q., Xu, X, Kang, Y, Gao, X., Huang, F., Wu, C., Ping, Y., “Genome editing of mutant KRAS through supramolecular polymer-mediated delivery of Cas9 ribonucleoprotein for colorectal cancer therapy.” J. Control Release, 2020 (322):236-247.

22. Gao, X., Lee, S.L. & Ding, N. “Single base editing tools with precise accuracy.” Provisional patent filed, 2019.

21. Hu, JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao, X., Rees HA, Lin Z, Liu DR., “Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.” Nature2018, 556(7699):57-63.

20. Gao, X., Tao Y., Lamas, V., Chen, Z. Y., Liu, D. R., et al, “Treatment of autosomal dominant hearing loss by in vivo delivery of genome editing agents.” Nature2018, 553, 217-221.

Before Rice:

19. Wang, M., Zuris, J. A., Meng, F., Rees H, Sun S, Deng P, Han Y, Gao, X., Liu, D. R., Xu, Q et al. “Efficient Delivery of Genome-Editing Proteins using Bioreducible Lipid Nanoparticles.”‎ Proc. Natl. Acad. Sci USA, 2016, 113(11):2868-73.

18. Jiménez-Osés, G., Osuna S., Gao, X., Tang, Y., Houk, K. N. et al, “The Role of Distant Mutations and Allosteric Regulation on LovD Active Site Dynamics.” Nat. Chem. Biol., 2014, (6):431-6.

17. Gao, X., Jiang, W, Houk K.N., Yi Tang, Walsh C.T. et al, “An Iterative, Bimodular Nonribosomal Peptide Synthetase that Converts Anthranilate and Tryptophan into Tetracyclic Asperlicins.” Chem. Biol., 2013, 20, 870-878.

16. Haynes, S. W., Gao, X., Tang, Y., Walsh, C. T., “Complexity Generation in Fungal Peptidyl Alkaloid Biosynthesis: a Two Enzyme Pathway to the Hexacyclic MDR Export Pump Inhibitor Ardeemin.” ACS Chem. Biol., 2013, 19;8(4):741-8.

15. Walsh, C. T., Haynes, S. W., Ames, B. D., Gao, X., Tang, Y. “Short Pathways to Complexity Generation: Fungal Peptidyl Alkaloid Multicyclic Scaffolds from Anthranilate Building Blocks.” ACS Chem. Biol., 2013, 19, 1366-82.

14. Wang, P., Bashiri, G., Sawaya, M. R., Gao, X., Tang, Y et al. “Uncovering the Enzymatic Basis of the Final Steps in Oxytetracycline Biosynthesis.” J. Am. Chem. Soc., 2013, 135, 7138-7141.

13. Gao, X., Haynes, S. W., Ames, B. D., Walsh, C. T., Tang, Y et al. “Cyclization of fungal nonribosomal peptides by a terminal condensation-Like domain.” Nat. Chem. Biol., 2012, 8, 823-830.

12. Wang, P., Gao, X., Tang, Y. “Redox enzymes to generate complexity of natural products.” Curr. Opin. Chem. Biol., 2012, 16, 362-369.

11. Wang, P., Kim, W., Pickens, L. B., Gao, X., Tang, Y. “Heterologous expression and manipulation of three tetracycline biosynthetic pathways.” Angew. Chem. Int. Ed., 2012, 51, 11136–11140.

10. Haynes, S. W., Gao, X., Tang, Y., Walsh, C. T. “Assembly of asperlicin peptidyl alkaloids from anthranilate and tryptophan: a two enzyme pathway generates heptacyclic scaffold complexity in asperlicin E.” J. Am. Chem. Soc., 2012, 134, 17444-17447.

9. Gao, X., Chooi, Y., Ames, B. D., Walsh, C. T., Tang, Y et al. “Fungal quinazoline alkaloid biosynthesis: Genetic and biochemical investigation of the tryptoquialanine pathway in Penicillium aethiopicum.” J. Am. Chem. Soc., 2011, 133, 2729-2741. (Highlighted by Nat. Chem. Biol.)

8. Wang, P., Gao, X., Chooi, Y., Deng, Z., Tang, Y. “Genetic characterization of enzymes involved in the priming steps of oxytetracycline biosynthesis in Streptomyces rimosus.” Microbiology, 2011, 157, 2401-2409.

7. Haynes, S. W., Ames, B. D., Gao, X., Tang, Y., Walsh, C. T. “Unraveling terminal C-domain-mediated condensation in fungal biosynthesis of imidazoindolone metabolites.” Biochemistry, 2011, 50, 5668-5679.

6. Yang, J., Yang, S., Gao, X., Yuan, Y.J. “Integrative investigation of lipidome and signal pathways in human endothelial cells under oxidative stress.” Mol. Biosyst., 2011, 7, 2428-2440.

5. Ames, B. D., Haynes, S. W., Gao, X., Tang, Y., Walsh, C. T. “Complexity generation in fungal peptidyl alkaloid biosynthesis: oxidation of fumiquinazoline A to the heptacyclic hemiaminal fumiquinazoline C by the flavoenzyme Af12070 from Aspergillus fumigatus.” Biochemistry, 2011, 50, 8392–8406.

4. Gao, X., Wang, P., Tang, Y. “Engineered polyketides biosynthesis and biocatalysis in Escherichia coli.” Appl. Microbiol. Biotechnol., 2010, 88, 1233-1242.

3. Gao, X., Xie, X., Pashkov, I., Sawaya, R. M., Tang, Y et al. “Directed evolution and structural characterization of a simvastatin synthase.” Chem. Biol., 2009, 16, 1064-1074. (2012 Presidential Green Chemistry Challenge Award)

2. Gao, X., Xie, X., Tang, Y. “LovD mutants exhibiting improved properties towards simvastatin synthesis.” U.S. Patent No. 8,981,056 (Licensed)

1. Xie, X., Pashkov, I., Gao, X., Yeates, T. O., Tang, Y et al. “Rational improvement of simvastatin synthase solubility in Escherichia coli leads to higher whole-cell biocatalytic activity.”  Biotechnol. Bioeng., 2009, 102, 20-28.